Journal: Oncotarget

Article Title: Chronic obstructive sleep apnea promotes aortic remodeling in canines through miR-145/Smad3 signaling pathway

doi: 10.18632/oncotarget.17144

Figure Lengend Snippet: (A) Representative bands of CollagenI and III and ratio of these proteins to GAPDH. (B) Representative bands of TGF-β1 and MMP9, data from these proteins were normalized to GAPDH. (C) Representative bands of phospho-Smad3 and Smad3 as well as the ratio of these proteins to GAPDH. * P < 0.05, * P < 0.01, ** * P <0.001 vs. sham group, n=6 in each group.

Article Snippet: After blocking with 5% nonfat dry milk, the membranes were then incubated with anti-CollagenI (bs-0578R, Bioss, China), anti-Collagen III (bs-0549R, Bioss, China), anti-TGFβ1 (ab92486, Abcam, USA), anti-α-SMA (ab5694, Abcam, USA), anti-MMP9 (bs-4593R, Bioss, China), anti-Smad3 (#9523, Cell Signaling Technology, USA), p-Smad3 (#9520, Cell Signaling Technology, USA), anti-AIF (ab1998, Abcam, USA), anti-cleaved caspase 3 (#9664, Cell Signaling Technology, USA), anti-caspase 9 (#9508, Cell Signaling Technology, USA), anti-Bcl-2 (ab32124, Abcam, USA), anti-Bax (ab32503, Abcam, USA), anti-GAP43 (ab12274, Abcam, USA), anti-TH (ab112, Abcam, USA), anti-NLRP3 (bs-10021R, Bioss, China), anti-NF-κB p65 (10745-1-AP, Proteintech, USA), anti-p-NF-κB p65 (#3033, Cell Signaling Technology, USA), anti-CD68 (ab76308, Abcam, USA), HIF-1α (R12-2180, Assaybiotech, USA), anti-GAPDH (KC-5G4, Kangchen, China) and anti-β-actin (TA-09, Zhongshan, China) antibodies in 4°C overnight.

Techniques: